The influence of local ultrasonic hyperthermia on toxic properties of cisplatin and doxorubicin

The aim of the study was to appreciate the influence of local ultrasonic hyperthermia on toxicological properties of cisplatin and doxorubicin. Material and methods. The experiments were performed on a total of 90 female outbreed rats (280-300 g) cisplatin (2,5 mg/kg/day) and doxorubicin (1,5 mg/kg/day) were injected intravenously into the caudal vein for 5 days with 24 h intervals 15 min before local ultrasonic hyperthermia. The site of thigh muscle of animals was heated for 10 min at mean 41,5 (±0,3) °C. Parameters for evaluation of toxicity: clinical signs including behavioral reactions, body weight, clinical and biochemical blood tests, urine analysis, electrocardiographic examination. Statistical analysis was performed by Microsoft Excel using Student's test. Internal organs and muscle of thigh from the site of local ultrasonic hyperthermic exposure were examined microscopically. Results. Local ultrasonic hyperthermia in regiment was used practically doesn't influence on clinical parameters of animals. Morphological changes of internal organs have, basically, reactive nature. Local tissue reactions are moderate. Combination of local ultrasonic hyperthermia with cisplatin or doxorubicin does not increase the toxicological properties of antineoplastic agents. Histopathological effects are equivalent in rats given anticancer drugs or their combinations with local ultrasonic hyperthermia. Conclusion. Combination of cisplatin or doxorubicin and local ultrasonic hyperthermia doesn't result in unexpected toxicities. By clinical application the control of combination toxicity, apparently, add up to the control of doxorubicin or cisplatin toxicity. The toxicity of cisplatin, while it combines with local hyperthermia, may become apparent later.

local ultrasonic hyperthermia, rats, cisplatin, doxorubicin, toxicity