Email this article Possibilies of application nanodiamond-based drug cariers for local chemotherapy. Experimental data
- Authors: Kurilchik A.A.1, Yuzhakov V.V.1, Konoplyannikov A.G.1, Bandurko L.N.1, Sevankaeva L.E.1, Filimonova M.V.1, Korchagina S.B.2, Gordeev S.K.2, Kiselev O.I.3, Tsyb A.F.1
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Affiliations:
- Federal State Budget Institution «Medical Radiological Research Center» of the Russian Ministry of Health
- Central Research Institute of Materials, Open Joint-Stock Company
- Federal State Budget Institution «Research Institute of Influenza» of the Russian Ministry of Health
- Issue: Vol 6, No 1 (2014)
- Pages: 64-69
- Section: EXPERIMENTAL ONCOLOGY
- Published: 27.01.2014
- URL: https://sarbon.abvpress.ru/jour/article/view/486
- ID: 486
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Abstract
Purpose. To study the effect of doxorubicin immobilized onto carbon nanostructured drug reservoirs on pathological morphology of experimental transplanted tumors.
Materials and methods. Experiments were performed on rats inoculated subcutaneously with sarcoma М-1 in the leg area. For anticancer drug delivery to the parenchyma of neoplasms we applied carbon reservoirs that had been fabricated on the basis of the nanodiamond composite and incubated in 0,01% and 0,1% doxorubicin solution. Immunohistochemical staining of proliferating cell nuclear antigen (PCNA) was performed in histological sections of sarcoma М-1.
Results. Histological examination revealed that around the drug reservoirs impregnated with 0,01% doxorubicin solution, there were narrow zones (0,1–0,35 mm wide) of tumor tissue destruction without the PCNA-positive reaction of cell nuclei. When the doxorubicin concentration increased by 10 times, the zones of the induced damage of М1 sarcoma appeared to be 3–4 times wider. In the tumor tissue adjoining to the areas of total destruction of cellular elements, a stromal edema, dissociation of tumor cells and their death in the mitotic phase were noted.
Conclusion. Doxorubicin-loaded carbon reservoirs implanted into the site of tumor growth produce a local alterative-anddestructive effect on experimental connective tissue tumors. The obtained results suggest that nanodiamond composites are promising for a selective delivery of chemotherapeutic agents to tumors.
About the authors
A. A. Kurilchik
Federal State Budget Institution «Medical Radiological Research Center» of the Russian Ministry of Health
Author for correspondence.
Email: Aleksandrkurilchik@yandex.ru
Obninsk
Russian FederationV. V. Yuzhakov
Federal State Budget Institution «Medical Radiological Research Center» of the Russian Ministry of Health
Obninsk
Russian FederationA. G. Konoplyannikov
Federal State Budget Institution «Medical Radiological Research Center» of the Russian Ministry of Health
Obninsk
Russian FederationL. N. Bandurko
Federal State Budget Institution «Medical Radiological Research Center» of the Russian Ministry of Health
Obninsk
Russian FederationL. E. Sevankaeva
Federal State Budget Institution «Medical Radiological Research Center» of the Russian Ministry of Health
Obninsk
Russian FederationM. V. Filimonova
Federal State Budget Institution «Medical Radiological Research Center» of the Russian Ministry of Health
Obninsk
Russian FederationS. B. Korchagina
Central Research Institute of Materials, Open Joint-Stock Company
St. Petersburg
Russian FederationS. K. Gordeev
Central Research Institute of Materials, Open Joint-Stock Company
St. Petersburg
Russian FederationO. I. Kiselev
Federal State Budget Institution «Research Institute of Influenza» of the Russian Ministry of Health
St. Petersburg
Russian FederationA. F. Tsyb
Federal State Budget Institution «Medical Radiological Research Center» of the Russian Ministry of Health
Obninsk
Russian FederationReferences
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