CRABP1 and CRABP2 expression in soft tissue sarcomas

Background. The study is devoted to the research of CRABP1 and CRABP2 mRNA as well as CRABP1 protein expression in human soft tissue sarcomas. Methods. Real time PCR, IHC analysis. Results. Here we for the first time studied the CRABP1 and CRABP2 genes expression by real time PCR in the samples of malignant mesenchymal tumors, including synovial sarcomas, malignant fibrous histiocytomas, malignant schwannomas and liposarcomas. We also analyzed CRABP1 protein level using immunohistochemistry in the samples of monophasic synovial sarcomas. Increase of CRABP1 and CRABP2 mRNA levels was revealed only in the synovial sarcomas and malignant fibrous histiocytomas samples (46% for CRABP1 and 54% for CRABP2). Opposite to synovial sarcomas and malignant fibrous histiocytomas, in the samples of malignant schwannomas and liposarcomas no elevation of CRABP1 or CRABP2 mRNA level was found; moreover, in 40% of these kind of tumors the decrease of CRABP1 mRNA level was observed. Immunohistochemical analysis revealed high level of CRABP1 protein in 100% of monophasic synovial sarcomas. Conclusion. The obtained results pointed on the differential CRABP1, -2 proteins expression in malignant soft tissue tumors depending on the histological type. The presented data open wide perspectives for further studies of CRABP1 as a potential prognostic marker and target for translational therapy of synovial sarcomas.

синовиальные саркомы, злокачественные фиброзные гистиоцитомы, липосаркомы, злокачественные шванномы, CRABP1, CRABP2, игх анализ, пЦР в реальном времени, synovial sarcomas, malignant fibrous histiocytomas, malignant schwannomas, liposarcomas, CRABP1, CRABP2, IHC analysis, real-time PCR