Analysis of anaplastic lymphoma kinase status in rhabdomyosarcomas: correlation with clinicopathological findings

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Abstract

Introduction. Rhabdomyosarcoma (RMS) is a highly malignant tumor occurring mostly in children. Multimodal therapy is currently promising, however, in patients with high risk (per the stratification scale) low survival rates are observed. There remains a necessity for new possibilities in treatment of this category of patients. Currently, anaplastic lymphoma kinase (ALK) is of considerable interest.

Aim. To study expression of ALK as a potential target marker in RMS tissues.

Materials and methods. In total, 202 histological RMS blocks were studied. ALK expression was qualitatively measured using immunohistochemistry, and its correlation with histological tumors subtype and patient’s clinical data was evaluated.

Results. ALK expression in samples of alveolar RMS was observed in 68 % (41/60) of cases, samples of embryonal RMS in 30 % (26/87) cases, samples of spindle cell/sclerosing RMS in 7 % (1/14) of cases, samples of epithelioid RMS (EpiRMS) in 100 % (2/2) of cases. The study showed correlation between ALK expression and alveolar RMS subtype (р <0.05). In all cases of alveolar RMS with focal ALK D5F3 expression, primarily low or moderate activity levels against anti-myogenin and MyoD1 were observed. ALK-positive samples in the embryonal and spindle cell/sclerosing RMS samples were rarer.

Conclusion. The results show that ALK is a promising target for treatment of patients with RMS. Further study of additional genetic aberrations of ALK is planned which could serve as a springboard for development of targeted therapy.

About the authors

D. Sh. Polatova

Tashkent State Dental Institute

Author for correspondence.
ORCID iD: 0000-0001-8128-2553

103 Makhtumkuli St., Tashkent 100047

Uzbekistan

G. K. Botiralieva

LLC “Ipsum pathology”

Email: gulrukhbotiralieva@gmail.com
ORCID iD: 0000-0001-6384-7645

1 Bogiston St., Tashkent 100058

Uzbekistan

D. M. Konovalov

Dmitry Rogachev National Medical Research center of Pediatric Hematology, Oncology and Immunology, Ministry of Health of Russia

ORCID iD: 0000-0001-7732-8184

1 Samora Mashela St., Moscow 117997

Russian Federation

B. X. Babanov

LLC “Ipsum pathology”

ORCID iD: 0000-0002-6760-0997

1 Bogiston St., Tashkent 100058

Uzbekistan

A. S. Sharlay

Dmitry Rogachev National Medical Research center of Pediatric Hematology, Oncology and Immunology, Ministry of Health of Russia

ORCID iD: 0000-0001-5354-7067

1 Samora Mashela St., Moscow 117997

Russian Federation

A. N. Muratov

LLC “Ipsum pathology”

1 Bogiston St., Tashkent 100058

Uzbekistan

S. A. Khayitboev

LLC “Ipsum pathology”

ORCID iD: 0000-0001-7470-2572

1 Bogiston St., Tashkent 100058

Uzbekistan

M. A. Zhakhongirova

Tashkent Medical Academy

ORCID iD: 0000-0002-7106-7128

2 Farobi St., Tashkent 100109

Uzbekistan

A. V. Savkin

Tashkent State Dental Institute

ORCID iD: 0000-0002-3416-5837

103 Makhtumkuli St., Tashkent 100047

Uzbekistan

N. K. Asamedinov

Tashkent State Dental Institute

ORCID iD: 0000-0001-5225-1539

103 Makhtumkuli St., Tashkent 100047

Uzbekistan

A. I. Nurzhabov

Tashkent State Dental Institute

ORCID iD: 0000-0002-8026-9944

103 Makhtumkuli St., Tashkent 100047

Uzbekistan

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Copyright (c) 2022 Polatova D.S., Botiralieva G.K., Konovalov D.M., Babanov B.X., Sharlay A.S., Muratov A.N., Khayitboev S.A., Zhakhongirova M.A., Savkin A.V., Asamedinov N.K., Nurzhabov A.I.

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